Liver response to dietary supplementation in bullfrog under stress condition

  • Jorgina Juliana Gradisse FREITAS Programa de Pós-graduação. Instituto de Pesca
  • Erna Elisabeth BACH Universidade Nove de Julho http://orcid.org/0000-0003-3238-673X
  • Isabella Cristina Antunes da Costa BORDON Programa de Pós-graduação. Instituto de Pesquisa de Energia Nuclear – IPEN
  • Ana Maria Cristina Rebello Pinto da Fonseca MARTINS Instituto Biológico
  • Marcio HIPOLITO Instituto Biológico http://orcid.org/0000-0002-1932-8829
  • Cláudia Maris FERREIRA Instituto de Pesca

Abstract

The hepatoprotective effect of dietary supplementation with the probiotic (Bacillus subtilis) and with beta-glucan on bullfrog in stress of high stocking density were evaluated . Animals were assigned in treatments: T1: 100 animals m-2 (control), T2: 236 animals m-2 and T3: 236 animals m-2, supplemented with probiotic; T4: 236 animals m-2, supplemented with beta-glucan. Survival, weight gain (WG) and the condition of liver biochemistry and histopathology were evaluated. There was no statistical difference in survival between treatments (P>0.05), but were observed significant differences for WG at 30 days, between unsupplemented treatments and supplemented treatments (P<0.001). Biochemical analysis showed conditions very near among animals of control treatment and animals supplemented with beta-glucan. Histopathological analysis revealed lesions and cytoplasmic rarefaction in 100% of the samples, suggesting protein deficiency. The beta-glucan reduced the effects of stress in bullfrog and has shown hepatoprotective effect, but the probiotic Bacillus subtilis was did not demonstrated the same effect.


 

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Published
2018-11-15
How to Cite
FREITAS, Jorgina Juliana Gradisse et al. Liver response to dietary supplementation in bullfrog under stress condition. Boletim do Instituto de Pesca, [S.l.], v. 40, n. 2, p. 261-269, nov. 2018. ISSN 1678-2305. Available at: <https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/1039>. Date accessed: 12 aug. 2022.
Section
Short Communication