Toxicity, freezing and cold storage tests of native species semen

  • Tiago Viana da COSTA Universidade Federal do Amazonas, Instituto de Ciências Sociais, Educação e Zootecnia
  • Lidia Miyako Yoshii OSHIRO Universidade Federal Rural do Rio de Janeiro, Estação de Biologia Marinha
  • Luciana Antunes de MATTOS Universidade Federal Rural do Rio de Janeiro, Estação de Biologia Marinha
  • Laura Susana LÓPEZ GRECO Universidad de Buenos Aires, Departamento de Biodiversidad y Biología Experimental
  • Emanuela Paula MELO Universidade Federal Rural do Rio de Janeiro, Estação de Biologia Marinha
  • Helaine dos Reis FLOR Universidade Federal Rural do Rio de Janeiro, Estação de Biologia Marinha


In the first part of this study, toxicity tests were performed on the sperm of Macrobrachium acanthurus using four cryoprotectants for periods of 10 and 20 min at concentrations of 10 and 20%. In the second part, cryopreservation was performed by applying the least toxic cryoprotectant, and two freezing methods were tested over 24 hours: automated (protocols A and B) and conventional (protocols C and D). Protocol A exhibited a cooling rate of 0.5°C min-1 from -6°C to -32°C; protocol B was similar to A except for the starting temperature, which was room temperature; whereas protocols C and D exhibited a cooling rate of 2 and 10°C min-1 respectively. The third part of the study was conducted to assess the lifespan of the sperm when stored at 5°C, in which sperm viability was evaluated by a semen smear with eosin-nigrosin. The least toxic cryoprotectants were 10 and 20% glycerol and 10% methanol, and the equilibrium time was 10 minutes. The optimal cooling rate was 2°C min-1 for 10% glycerol, which had a sperm survival rate of 21.8%. Cold storage for up to 3 days is recommended, presenting a sperm survival rate of 35.3%.


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How to Cite
COSTA, Tiago Viana da et al. Toxicity, freezing and cold storage tests of native species semen. Boletim do Instituto de Pesca, [S.l.], v. 43, n. 3, p. 334-346, sep. 2017. ISSN 1678-2305. Available at: <>. Date accessed: 23 may 2022. doi:

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