Nematode and cestode larvae of hygienic-sanitary importance in Lopholatilus villarii (Actinopterygii) in the State of Rio de Janeiro, Brazil

  • Aline Monteiro da SILVA Laboratório de Inspeção e Tecnologia de Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense / Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, FIOCRUZ
  • Marcelo KNOFF Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, FIOCRUZ http://orcid.org/0000-0003-1934-5642
  • Nilza Nunes FELIZARDO Laboratório de Inspeção e Tecnologia de Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense
  • Delir Corrêa GOMES Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, FIOCRUZ
  • Sérgio Carmona de SÃO CLEMENTE Laboratório de Inspeção e Tecnologia de Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense

Abstract

The tile fish, Lopholatilus villarii, occurs from Brazil to Argentina where it is commercially important because it possesses highly appreciated meat. The aim of this study was to identify the larvae of anisakid and raphidascaridid nematodes and trypanorhynch cestodes in L. villarii purchased in fish markets of the municipality of Niterói, state of Rio de Janeiro, Brazil, and calculate their parasitological indices and present their sites of infection. From August 2015 to September 2016, 31 specimens of L. villarii were investigated. The fish were necropsied and their viscera and musculature, analyzed. The helminths found were processed according to standard helminthological techniques for taxonomic identification. Of 31 fish analyzed, 28 (90.3%) were parasitized with larvae of anisakid and raphidascaridid nematodes: Anisakis sp., Terranova sp., Pseudoterranova sp. and Hysterothylacium deardorffoverstreetorum (L3 and L4) and plerocercus of the trypanorhynch cestode Otobothrium sp. Their parasitological indices were calculated. The helminths parasitized mainly the serosa of intestine, stomach and liver, and H. deardorffoverstreetorum was also parasitizing the abdominal musculature of one fish. This is the first report of anisakid and raphidascaridid nematodes and trypanorhynch cestodes in L. villarii. Hygienic-sanitary aspects were discussed.

References

ACHA, P.N.; SZYFRES, B. 2003 Zoonoses and communicable diseases common to man and animals.3º ed. Washington, D.C.: PAHO. 395 p.

ADAMS, A.M.; MURREL, K.D.; CROSS, J.H. 1997 Parasites of fish and risk to public health.Scientifical and Technical Review of the Office International des Epizooties, 16(2): 652-660.

ALVES, D.R.; LUQUE, J.L.; PARAGUASSÚ, A.R. 2002 Community ecology of the metazoan parasites of pink cusk-eel, Genypterus brasiliensis (Osteichthyes: Ophidiidae), from the coastal zone of the state of Rio de Janeiro. Memórias do Instituto Oswaldo Cruz, 97(5): 683-689.

ALVES, D.R.; PARAGUASSÚ, A.R.; LUQUE, J.L. 2005 Community ecology of the metazoan parasites of the grey triggerfish, Balistes capriscus Gmelin, 1789 and queen triggerfish B. vetula Linnaeus, 1758 (Osteichthyes: Balistidae) from the State of Rio de Janeiro, Brazil. Revista Brasileira de Parasitologia Veterinária, 14(2): 71-77.

AMATO, J.F.R.; SÃO CLEMENTE, S.C.; OLIVEIRA, G.A. 1990 Tentacularia coryphaenae Bosc, 1801
(Eucestoda: Trypanorhyncha) in the inspection and technology of the Skipjack Tuna, Katsuwonus
pelamis (L.) (Pisces: Scombridae). Atlântica, 12(1):73-77.

AUDICANA, M.T.; ANSOTEGUI, I.J.; CORRES, L.F.; KENEDDY, M.W. 2002 Anisakis simplex:
dangerous – dead and alive? Trends in Parasitology, 18(1): 20-25.

BEVERIDGE, I.; JUSTINE, J.L. 2007 Redescriptions of four species of Otobothrium Linton, 1890
(Cestoda: Trypanorhyncha), including new records from Australia, New Caledonia and
Malaysia, with the description of O. parvum n.sp. Zootaxa, 1587: 1–25.

BORGES, J.; CUNHA, L.; SANTOS, H.; MONTEIRONETO, C.; SANTOS, C. 2012 Morphological and molecular diagnosis of anisakid nematode larvae from cutlassfish (Trichiurus lepturus) off
the coast of Rio de Janeiro, Brazil. PLoS ONE, 7(7): e40447.

BUSH, A.O.; LAFFERTY, K.D.; LOTZ, J.M.; SHOSTAK, A.W. 1997 Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology, 83(4): 575-583.

CAMPBELL, R.A.; BEVERIDGE, I. 1994 Order Trypanorhyncha Diesing, 1863. In: KHALIL,L.F.; JONES, A.; BRAY, R.A. (Eds.). Keys to the cestodes parasites of vertebrates. Cambridge: CABI.p.51148.

CHAI, J.Y.; MURRELL, K.D.; LYMBERY, A.J. 2005 Fish-borne parasitic zoonoses: status and issues.
International Journal for Parasitology, 35(11-12): 1233-1254.

CRUZ, A.R.; SOUTO, P.C.S.; FERRARI, C.K.B.; ALLEGRETTI, S.M.; ARRAIS-SILVA, W.W. 2010 Endoscopic imaging of the first clinical case of Anisakidosis in Brazil. Scientia Parasitologica,
11(2): 97-100.

D’AMICO, P.; MALANDRA, R.; COSTANZO, F.; CASTIGLIEGO, L.; GUIDI, A.; GIANFALDONI,D.; ARMANI., A. 2014 Evolution of the Anisakis risk management in the European and Italian contexto. Food Research International, 64(1): 348-362.

DIAS, F.J.E.; SÃO CLEMENTE, S.C.; KNOFF, M. 2010 Nematoides anisaquídeos e cestoides
Trypanorhyncha de importância em saúde pública em Aluterus monoceros (Linnaeus, 1758)
no Estado do Rio de Janeiro, Brasil. Revista Brasileira de Parasitologia Veterinária, 19(2); 94-97.

DIAS, F.J.E.; SÃO CLEMENTE, S.C.; PINTO, R.M.; KNOFF, M. 2011 Anisakidae nematodes and
Trypanorhyncha cestodes of hygienic importance infecting the king mackerel Scomberomorus
cavalla (Osteichthyes: Scombridae) in Brazil. Veterinary Parasitology, 175(3-4): 351-355.

FELIZARDO, N.N; KNOFF, M.; PINTO, R.M;GOMES, D.C. 2009 Larval anisakid nematodes of the flounder Paralichthys isosceles Jordan,1890 (Pisces: Teleostei) from Brazil. Neotropical Helminthology, 3(2): 57-64.

FELIZARDO, N.N.; TORRES, E.J.L.; FONSECA, M.C.G.; PINTO, R.M.; GOMES, C.D.;KNOFF, M. 2010 Cestodes of the flounder Paralichthys isosceles Jordan, 1890 (Osteichthyes -Paralichthyidae) from the State of Rio de Janeiro,Brazil. Neotropical Helminthology, 4(2): 113-125.

FIGUEIREDO, J.L.; MENEZES, N.A. 1980 Manual de peixes marinhos do Sudeste do Brasil. III. Teleostei (2). São Paulo: Universidade de São Paulo,Museu de Zoologia. 93 p.

FONSECA, M.C.G.; KNOFF, M.; FELIZARDO,N.N.; DI AZEVEDO, M.I.N.; LOPES TORRES,E.J.; GOMES, D.C.; IÑIGUEZ, A.M.; SÃO CLEMENTE, S.C. 2016 Integrative taxonomy of Anisakidae and Raphidascarididae (Nematoda) in Paralichthys patagonicus and Xystreurys rasile (Pisces: Teleostei) from Brazil. International Journal of Food Microbiology, 235(1): 113–124.

FONTENELLE, G.; KNOFF, M.; FELIZARDO,N.N.; LOPES, L.M.S.; SÃO CLEMENTE, S.C. 2013 Nematodes of zoonotic importance in Cynoscion guatucupa (Pisces) in the state of Rio de Janeiro. Revista Brasileira de Parasitologia Veterinária, 22(2): 281-284.

FONTENELLE, G.; KNOFF, M.; FELIZARDO, N.N.; LOPES TORRES, E.J.; LOPES, L.M.S.; GOMES,
D.C.; SÃO CLEMENTE, S.C. 2015 Anisakidae and Raphidascarididae larvae parasitizing Selene
setapinnis (Mitchill, 1815) in the State of Rio de Janeiro, Brazil. Brazilian Journal of Veterinarian
Parasitology, 24(1): 72-77.

FROESE, R.; PAULY, D. 2016 FishBase. World Wide Web electronic publication. Available from:<
http://www.fishbase.org/summary/15383>Access in: Nov. 28 2016.

GÒMEZ-MORALES, M.A; LUDOVISI, A.; GIUFFRA, E.;MANFREDI, M.T.; PICCOLO, G.; POZIO, E. 2008
Allergenic activity of Molicola horridus (Cestoda,Trypanorhyncha), a cosmopolitan fish parasite, in amouse model. Veterinary Parasitology, 157(3-4): 314-320.

HURST, R.J. 1984 Identification and description of larval Anisakis simplex and Pseudoterranova
decipiens (Anisakidae: Nematoda) from New Zealand waters. New Zealand Journal of Marine
and Freshwater Research, 18(2): 177-186.

KNOFF, M.; GOMES, D.C. 2012 Capítulo 3. Metodologia básica para coleta e processamento
de helmintos parasitos. In: MOLINARO, E.; CAPUTO, L.; AMENDOEIRA, R. Conceitos e Métodos para formação de profissionais em laboratórios de saúde: volume 5. Ed. Escola Politécnica de Saúde Joaquim Venâncio, Fundação Oswaldo Cruz, Rio de Janeiro. p. 251-281.

KNOFF, M.; FELIZARDO, N.N.; IÑIGUEZ, A.M.; MALDONADO, A.J.R.; TORRES, E.J.L.; PINTO, R.M.; GOMES, D.C. 2012 Genetic and morphological characterisation of a new species of the genus Hysterothylacium (Nematoda) from Paralichthys isosceles Jordan, 1890 (Pisces: Teleostei) of the Neotropical region, state of Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, 107(2): 186-193.

KNOFF, M.; SÃO CLEMENTE, S.C.; FONSECA, M.C.G.; ANDRADA, C.G.; PADOVANI, R.E.S.; GOMES, D.C. 2007 Anisakidae parasitos de congro-rosa, Genypterus brasiliensis Regan, 1903 comercializados no estado do Rio de Janeiro, Brasil de interesse na saúde pública. Parasitologia
Latinoamericana, 62(3-4): 127-133.

KNOFF, M.; SÃO CLEMENTE, S.C.; FONSECA, M.C.G.; FELIZARDO, N.N.; LIMA, F.C.; PINTO, R.M.; GOMES, D.C. 2013. Anisakidae nematodes in the blackfin goosefish, Lophius gastrophysus
Miranda-Ribeiro, 1915 purchased in the State of Rio de Janeiro, Brazil. Acta Scientiarum. Biological
Sciences, 35(1): 129-133.

KURAIEM, B.P.; KNOFF, M.; FELIZARDO, N.N.; GOMES, D.C.; SÃO CLEMENTE, S.C. 2016 Nematode larvae infecting Priacanthus arenatus Cuvier, 1829 (Pisces: Teleostei) in Brazil. Anais da Academia Brasileira de Ciências, 88(2): 857-863.

LIMA, F.C; MESQUITA, E.F.M. 1996 Fraudes detectadas na comercialização de pescado no município de Niterói, Estado do Rio de Janeiro, Brasil. Revista Brasileira de Ciências Veterinária, 3(2): 39-43.

LOPES TORRES, E.J; SOUZA, W. MIRANDA, K.2013 Comparative analysis of Trichuris muris surface using conventional, low vacuum,environmental and field emission scanning electron microscopy. Veterinary Parasitology,196(3-4): 409-416.

LUQUE, J.L.; ALVES, D.R. 2001 Ecologia das comunidades de metazoários parasitos, do xaréu, Caranx hippos (Linnaeus) e do xerelete, Caranx tatus Agassiz (Osteichthyes, Carangidae)
do litoral do estado do Rio de Janeiro, Brasil. Revista Brasileira de Zoologia, 18(2): 399 - 410.

MAFRA, C.; MANTOVANI, C.; BORGES, J.N.; BARCELOS, R. M.; SANTOS, C.P. 2015 Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil. Brazilian Journal of Veterinary Parasitology, 24(2): 209-215.

MATTOS, D.P.B.G; VERÍCIMO, M.A.; SÃO CLEMENTE, S.C. 2013 O Pescado e os Cestoides Trypanorhyncha – Do Aspecto Higiênico ao potencial alergênico. Veterinária Notícias, 19(2): 127-139.

MCCLELLAND, G. 1980 Phocanema decipiens. Molting in seals. Experimental Parasitology, 49(1):
128-136.

PAIVA, M.P.; ANDRADE-TUBINO, M.F. 1998 Distribuição e abundância de peixes bentônicos
explotados pelos linheiros ao largo do sudeste do Brasil (1986-1995). Revista Brasileira de
Biologia, 58(4): 619-632.

PALM, H.W. 1997 Trypanorhynch cestodes of commercial fishes from northeast Brazilian coastal waters. Memórias do Instituto Oswaldo Cruz, 92(1): 69–79.

PALM, H.W. 2004 The Trypanorhyncha Diesing, 1863. Bogor: PKSPL-IPB Press. 710 p.

PALM, H.W; MUNDT, U.; OVERSTREET, R. 2000 Sensory receptors and surface ultrastructure
within trypanorhynch cestodes. Parasitology Research, 86(10): 821–833.

PALM, H.; OBIEKEZIE, A.; MÖLLER, H. 1994

Trypanorhynchid cestodes of commercial inshore fishes of the West African coast. Aquatic
Living Resources, 7(3): 153-164.

PANTOJA, C.S.; BORGES, J.N.; SANTOS, C.P.;LUQUE, J.L. 2015 Molecular and morphological
characterization of anisakid nematode larvae from the sandperches Pseudopercis numida
and Pinguipes brasilianus (Perciformes: Pinguipedidae) off Brazil. Journal of Parasitology,
101(4): 492–499.

PETTER, J.A.; MAILLARD, C. 1988 Larves d’ascarides parasites de poissons em Méditerranée
Occidentale. The Bulletin of the Natural History Museum, 10(2): 347-369.

RIBEIRO, J.; SÃO CLEMENTE, S.C.; LOPES, L.M.S.; KNOFF, M. 2014 Nematode larvae of hygienic
importance infecting Chaetodipterus faber (Broussonet, 1782) and Trachinotus carolinus
(Linnaeus, 1766) (Pisces: Teleostei) in Brazil. Revista Brasileira de Medicina Veterinária, 36(2):
121-124.

RODERO, M.; CUÉLLAR, C. 1999 Humoral immune responses induced by Gymnorhynchus gigas
extracts in BALB/c mice. Journal of Helminthology, 73(3): 239-243.

SAAD, C.D.R.; VIEIRA, F.M.; LUQUE, J.L. 2012 Larvae of Anisakidae Skrjabin & Karokhin, 1945
(Nematoda, Ascaridoidea) in Lophius gastrophysus Miranda-Ribeiro, 1915 (Actinopterygii,
Lophiidae) from the coastal zone of the state of Rio de Janeiro, Brazil. Neotropical Helminthology,
6(2): 159-177.

SÃO CLEMENTE, S.C.; KNOFF, M.; PADOVANI, .E.S.; LIMA, F.C.; GOMES, D.C. 2004 Cestóides Trypanhorhyncha parasitos de congro-rosa, Genypterus brasiliensis, Regan,1903 comercializados nos municípios de Niterói e rio de Janeiro, Brasil. Revista Brasileira de Parasitologia Veterinária, 13(3): 97-102.

SÃO CLEMENTE, S.C.; KNOFF, M.; LIMA, F.C.; ANDRADA, C.D.G.; FELIZARDO, N.N.; PADOVANI, R.E.S.; GOMES, D.C. 2007 Cestóides Trypanorhyncha parasitos de peixe sapo-pescador, Lophius gastrophysus Miranda- Ribeiro, 1915 comercializados no Estado do Rio de Janeiro, Brasil. Revista Brasileira de Parasitologia Veterinária, 16(1): 37-42.

SÃO CLEMENTE, S.C; LIMA, F.C; UCHOA, C.M.1995 Parasitos de Balistes vetula (L.) e sua importância na inspeção do pescado. Revista Brasileira de Ciência Veterinária, 2(2): 39-41.

SCHAEFFNER, B.C.; BEVERIDGE, I. 2013 Redescriptions and new records of species of Otobothrium Linton, 1890 (Cestoda: Trypanorhyncha). Systematic Parasitology, 84(1):17-55.

TAVARES, L.E.R.; BICUDO, A.J.A.; LUQUE, J.L. 2004 Metazoan parasites of needlefish Tylosurus
acus (Lacépède, 1803) (Osteichthyes: Belonidae) from the coastal zone of the state of Rio de
Janeiro, Brazil. Revista Brasileira de Parasitologia Veterinaria, 13(1): 36-40.

TIMI, J.T; SARDELLA, N.H; NAVONE, G.T. 2001 Parasitic nematodes of Engraulis anchoita Hubbs
et Marini, 1935 (Pisces, engraulidae) of the Argentine and Uruguayan coasts, South West Atlantic. Acta Parasitologica, 46(3): 186-193.

VÁZQUEZ-LÓPEZ, C.; ARMAS-SERRA, C.; BERNARDINA, W.; RODRÍGUEZ-CAABEIRO, F. 2001 Oral inoculation with Gymnorhynchus gigas induces anti-parasite anaphylatic antibody production in both mice and rats and adverse reactions in challenge mice. International Journal of Food Microbiology, 64(3): 307-315.

VÁZQUEZ-LÓPEZ, C.; ARMAS-SERRA, C.; BERNARDINA, W.; RODRÍGUEZ-CAABEIRO, F. 2002 A 24-kDa collagenase from Gymnorhynchus gigas elicits rat ileum hyperreactivity and is a target of humoral responses in mice previously given a single oral dose of parasite extract.
Digestive Diseases and Sciences, 47(4): 935-942.
Published
2017-09-25
How to Cite
SILVA, Aline Monteiro da et al. Nematode and cestode larvae of hygienic-sanitary importance in Lopholatilus villarii (Actinopterygii) in the State of Rio de Janeiro, Brazil. Boletim do Instituto de Pesca, [S.l.], v. 43, n. 3, p. 385–398, sep. 2017. ISSN 1678-2305. Available at: <https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/1213>. Date accessed: 25 may 2022. doi: https://doi.org/10.20950/1678-2305.2017v43n3p385.

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