Clinostomum sp. (Digenea: Clinostomidae) and Ascocotyle sp. (Digenea: Heterophyidae): metacercariae with zoonotic potential in fishes from Tramandaí River basin, southern Brazil

  • Alice POZZA Universidade do Vale do Rio dos Sinos – UNISINOS, Laboratório de Ictiologia
  • Fábio LIMA Universidade do Vale do Rio dos Sinos – UNISINOS, Laboratório de Ictiologia
  • Mateus Luís HAAS Universidade do Vale do Rio dos Sinos – UNISINOS, Laboratório de Ictiologia
  • Pablo Cesar LEHMANN ALBORNOZ Universidade do Vale do Rio dos Sinos – UNISINOS, Laboratório de Ictiologia http://orcid.org/0000-0002-8884-4827

Abstract

 The helminths belonging to the class Trematoda have great importance in ichthyoparasitology, and the subclass Digenea includes parasites with zoonotic potential. The family Heterophyidae is responsible for the heterophyiasis and, in Brazil, this disease is caused by the trematode genera Ascocotyle (Phagicola), commonly known as phagicolosis, both related to the fish-to-birds/mammals cycle. Clinostomum (belonging to the family Clinostomidae) has its larval stage in fish musculature and piscivorous birds as definitive hosts. It may parasitize humans accidentally, causing parasitic laryngopharyngitis and death by asphyxia. This study aims to quantify the presence of digenetic Heterophyidae and Clinostomidae parasites with zoonotic potential in fish from the Tramandaí River basin, in the state of Rio Grande do Sul, Brazil. The fish were purchased from fishermen from Terra de Areia/RS, from July 2016 to May 2017. The search for parasites was carried out by performing a necropsy on the fish. The digenetic parasites were preserved, stained and mounted on microscope slides for identification. Parasitological indices were calculated. Astyanax spp. Were positive for Ascocotyle whereas Geophagus brasiliensis was infected by Clinostomum sp. Therefore, the results suggest a risk of infection to the consumer population in the region.

References

ALMEIDA FILHO, E.S.; VALENTE, A.M.; STUSSI, J.S.P.; OLIVEIRA, L.A.T.; FRANCO, R.M.; CARVALHO, J.C.A.P. 2004 Vibrio vulnificus em pescado, uma revisão. Higiene Alimentar, 18(116/117): 23-28.

AMATO, J.F.R.; AMATO, S.B. 2010 Técnicas gerais para coleta e preparação de helmintos endoparasitos de aves. In: VON MATTER, S.; STRAUBE, F.C.; ACCORDI, I.A.; PIACENTINI, V.Q.; CÂNDIDO JUNIOR, J.F. Ornitologia e conservação: ciência aplicada, técnicas de pesquisa e
levantamento. Rio de Janeiro: Technical Books. p. 369-393.

BARROS, L.A.; AMATO, S.B. 1995 Aspectos patológicos observados em hamsters (Mesocricetus auritus) infectados experimentalmente com metacercárias de Phagicola longus (Ranson, 1920) Price, 1932 (Digenea, Heterophyidae). Revista Brasileira de Parasitologia Veterinária, 4(1): 43-48.

BELIZARIO JUNIOR, V.Y.; DE LEON, W.U.; BERSABE, M.J.; PURNOMO; BAIRD, J.K.; BANGS, M.J. 2004 A focus of human infection by Haplorchis taichui (Trematoda: Heterophyidae) in the southern Philippines. The Journal of Parasitology, 90(5): 1165-1169. PMid:15562620. http://dx.doi.org/10.1645/GE-3304RN.

BRASIL, MINISTÉRIO DA SAÚDE. 2010 Classificação de risco dos agentes biológicos. 2ª ed. Brasília: Ministério da Saúde. 44p. (Série A. Normas e Manuais Técnicos).

BUSH, A.O.; LAFFERTY, K.D.; LOTZ, J.M.; SHOSTAK, A.W. 1997 Parasitology meests ecology on its own terms: Margolis et al. 1982. Revisited. Journal of Parasitology, 83(4): 575-583. http://dx.doi.
org/10.2307/3284227.

CAFFARA, M.; LOCKE, S.A.; GUSTINELLI, A.; MARCOGLIESE, D.J.; FIORAVANTI, M.L. 2011 Morphological and molecular differentiation of Clinostomum complanatum and Clinostomum marginatum (Digenea: Clinostomidae) metacercariae and adults. The Journal of Parasitology,
97(5): 884-891. PMid:21711099. http://dx.doi.org/10.1645/GE-2781.1.

CAMERON, T.W.M. 1945 Fish-carried parasites in Canada. I—Parasites carried by freshwater fish. Canadian Journal of Comparative Medicine, 9(9): 245-254. PMid:17648150.

CARVALHO, A.R.; TAVARES, L.E.R.; LUQUE, J.L. 2008 Metacercárias tipo Neascus em Geophagus brasiliensis (Perciformes: Cichlidae) do rio do Peixe, Juiz de Fora, Brasil. Acta Scientiarum. Biological Sciences, 30(3): 315-320.

CHAI, J.-Y.; MURRELL, K.D.; LYMBERY, A.J. 2005 Fish-borne parasitic zoonoses: status and issues. International Journal for Parasitology, 35(11-12): 1233-1254. PMid:16143336. http://dx.doi.org/10.1016/j.ijpara.2005.07.013.

CHENG, T.C. 2012 General Parasitology. 2ª ed. New York, NY: Academic Press. 788p.

CHIEFFI, P.P.; GORLA, M.C.; TORRES, D.M.; DIAS, R.M.; MANGINI, A.C.; MONTEIRO, A.V.; WOICIECHOVSKI, E. 1992 Human infection by Phagicola sp. (Trematoda: Heterophyidae) in the municipality of Registro, São Paulo State, Brazil. The Journal of Tropical Medicine and Hygiene, 95(5): 346-348. PMid:1404559.

CHIEFFI, P.P.; LEITE, O.H.; DIAS, R.M.D.S.; TORRES, D.M.A.V.; MANGINI, A.C.S. 1990 Human parasitism by Phagicola longa (Trematoda: Heterophyidae) in Cananéia, São Paulo State, Brazil.
Revista do Instituto de Medicina Tropical de Sao Paulo, 32(4): 285-288. PMid:2101522. http://dx.doi.org/10.1590/S0036-46651990000400008.

CHUNG, D.I.; KONG, H.H.; JOO, C.Y. 1998 Radix auricularia coreana: natural snail host of Clinostomum complanatum in Korea. Korean Journal of Parasitology, 36(1): 1-6. PMid:9529857. http://dx.doi.org/10.3347/kjp.1998.36.1.1.

CITTI, A.L. 2012 Tainhas (Mugil liza, Valenciennes, 1836) infectadas por Ascocotyle (Phagicola) longa em São Paulo: ocorrência, importância na saúde pública, estratégias de controle e sua representatividade cultural. São Paulo. 124f. (PhD Thesis. USP). Available from: www.teses.usp.br/teses/disponiveis/10/10134/tde-26072012-132239/en.php>. Access on: 13 july 2017

DIAS, J.S.; POZZA, A.; PESENTI, T.C.; PEREIRA JUNIOR, J.; BERNE, M.E.A., 2016 Helmintos parasitos de Rhamdia quelen (Q uoy & Gaimard, 1824) no sul do Brasil. Science and Animal Health, 4(1): 2-20. EIRAS, J.C. 1994 Elementos de Ictioparasitologia. 1ª ed. Porto, PT: Fundação Eng. Antônio de Almeida. 339p.

GIBSON, D.I.; JONES, A.; BRAY, R.A. 2002 Keys to the trematoda. Wallingford, UK: CABI Publishing and The Natural History Museum. v. 1, 544p. HARA, H.; MIYAUCHI, Y.; TAHARA, S.; YAMASHITA, H. 2014 Human laryngitis caused by Clinostomum complanatum. Nagoya Journal of Medical Science, 76(1-2): 181-185. PMid:25130004.

HIRAL, H.; OOISO, H.; KIFUNE, T.; KIYOTA, T.; SAKAGUCHI, Y. 1988 Clinostomum complanatum infection in posterior wall of the pharynx of a human. Japanese Journal of Parasitology, 36(3): 142-144.

JACKSON, G.J.; BIER, J.W. 1981 Review of human anisakiasis. Rome: FDA By-Lines. v. 3, p. 152-156.

KITAGAWA, N.; ODA, M.; TOTOKI, T.; WASHIZAKI, S.; ODA, M.; KIFUNE, T. 2003 Lidocaine spray used to capture a live Clinostomum parasite causing human laryngitis. American Journal of Otolaryngology, 24(5): 341-343. PMid:13130449. http://dx.doi.org/10.1016/S0196-0709(03)00060-7.

LIZAMA, M.L.A.P.; TAKEMOTO, R.M.; PAVANELLI, G.C. 2008 Ecological aspects of metazoan parasites of Astyanax altiparanae Garutti e Britski, 2000 (Characidae) of the upper Paraná river floodplain, Brazil. Boletim do Instituto de Pesca, 34(4): 527-533.

LUQUE, J.L. 2004 Biologia, epidemiologia e controle de parasitos de peixes. Revista Brasileira de Parasitologia Veterinária, 13(suppl. 1): 161-164.

MADI, R.R. 2005 Utilização dos helmintos parasitos de Geophagus brasiliensis (Quoy & Gaimard, 1824) (Cichlidae; Perciformes) como indicadores ambientais. Campinas. 110f. (PhD Thesis. UNICAMP). Available from: .
Access on: 24 july 2017.

MALABARBA, L.R.; CARVALHO NETO, P.; BERTACO, V.A.; CARVALHO, T.P.; SANTOS, J.F.; ARTIOLI, L.G.S. 2013 Guia de identificação dos peixes da bacia do rio Tramandaí. 1ª ed. Porto Alegre: Via Sapiens. 140p.

MARQUES, M.C.; CLEMENTE, S.C.; BAROS, H.C. 1995 Utilização do frio (resfriamento e congelamento) na sobrevivência de larvas de nematóides anisakídeos em Trichiurus lepturus. Higiene Alimentar, 9(39): 23-28.

MYERS, B.J. 1976 Research then and now on the Anisakidae nematodes. Transactions of the American Microscopical Society, 95(2): 137-142. PMid:775719. http://dx.doi.org/10.2307/3225056.

NEVES, D.P. 2009 Parasitologia dinâmica. 3ª ed. Rio de Janeiro: Atheneu Editora. 591p.

OLIVEIRA, S.A.; HERNANDEZ-BLAZQUEZ, F.J.; ANTUNES, S.A.; MAIA, A.A.M. 2007 Metacercárias de Ascocotyle (Phagicola) longa Ransom, 1920 (Digenea: Heterophyidae), em Mugil platanus, não
Estuário de Cananéia, SP, Brasil. Ciência Rural, 37(4): 1056-1059. http://dx.doi.org/10.1590/S0103-84782007000400022.

PARAGUASSÚ, A.R.; ALVES, D.R.; LUQUE, J.L. 2005 Metazoários parasitos do acará Geophagus brasiliensis (Quoy; Gaimard, 1824) (Osteichthyes: Cichlidae) do reservatório de Lajes, estado do Rio de Janeiro, Brasil. Revista Brasileira de Parasitologia Veterinária, 14(1): 35-39. PMid:16153342.

PARK, C.W.; KIM, J.S.; JOO, H.S.; KIM, J.A. 2009 Human Case of Clinostomum complanatum infection in Korea. Korean Journal of Parasitology, 47(4): 401-404. PMid:19967090. http://dx.doi.
org/10.3347/kjp.2009.47.4.401.

SALGADO-MALDONADO, G.; AGUILAR-AGUILAR, R.; CABAÑASCARRANZA, G.; SOTO-GALERA, E.; MENDOZA-PALMERO, C. 2005 Helminth parasites in freshwater fish from the Papaloapan River
basin, Mexico. Parasitology Research, 96(2): 69-89. PMid:15812673.
http://dx.doi.org/10.1007/s00436-005-1315-9.

SCHOLZ, T.; AGUIRRE-MACEDO, M.L.; SALGADO-MALDONADO,
G. 2001 Trematodes of the family Heteropyidae (Digenea) in Mexico: a review of species and new host and geographical records. Journal of Natural History, 35(12): 1733-1772. http://dx.doi.
org/10.1080/00222930152667087.

SCHOLZ, T.; VARGAS-VÁZQUEZ, J.; MORAVEC, F.; VIVAS-RODRÍGUEZ, C.; MENDOZA-FRANCO, E. 1995 Metacercariae of trematodes of fishes from cenotes (= sinkholes) of the Yucatan Peninsula, Mexico. Folia Parasitologica, 42(3): 173-192.

SCHOLZ, T.; VARGAS-VÁZQUEZ, J.; VIDAL-MARTÍNEZ, V.M.; AGUIRRE-MACEDO, L. 1997 Ascocotyle nunezae n. sp. (Digenea: Heterophyidae), from Yucatan, Mexico. The Journal of Parasitology,
83(1): 141-147. PMid:9057711. http://dx.doi.org/10.2307/3284333.

SILVA, A.S.; MONTEIRO, S.G.; DOYLE, R.L.; PEDRON, F.A.; FILIPETTO, J.E.; RADUNZ-NETO, J. 2008 Ocorrência de Clinostomum complanatum em diferentes espécies de peixes de uma piscicultura do Município de Santa Maria - RS. Veterina-ria e Zootecnia, 15(1): 27-32.

SUTILI, F.J.; GRESSLER, L.T.; PELEGRINI, L.F.V. 2014 Clinostomum complanatum (Trematode, Digenea): A parasite of birds and fishes with zoonotic potential in southern Brazil: A review. Revista Brasileira de Higiene e Sanidade Animal, 8(1): 99-114. http://dx.doi.org/10.5935/1981-2965.20140007.

TAKEMOTO, R.M.; PAVANELLI, G.C.; LIZAMA, M.A.P.; LACERDA, A.C.F.; YAMADA, F.H.; MOREIRA, L.H.A.; CESCHINI, T.L.; BELLAY, S. 2009 Diversity of parasites of fish from the Upper Paraná River
floodplain, Brazil. Brazilian Journal of Biology = Revista Brasileira de Biologia, 69(2, suppl. ): 691-705. PMid:19738975. http://dx.doi.org/10.1590/S1519-69842009000300023.

TIEWCHALOERN, S.; UDOMKIJDECHA, S.; SUVOUTTHO, S.; CHUNCHAMSRI, K.; WAIKAGUL, J. 1999 Clinostomum trematode from human eye, the Southeast Asian. Journal of Tropical Medicine
and Public Health, 30(2): 382-384.

VIOLANTE-GONZÁLEZ, J.; AGUIRRE-MACEDO, M.L.; MENDOZAFRANCO, E.F. 2007 A checklist of metazoan parasites of fish from Tres Palos Lagoon, Guerrero, Mexico. Parasitology Research, 102(1): 151-161. PMid:17846791. http://dx.doi.org/10.1007/s00436-007-0733-2.

WITENBERG, G. 1944 What is the cause of the parasict laryngo-pharyngitis in the Near East (“Halzoun”)? Acta Medica Orientalia, 3(6): 191-192.

YAMASHITA, J. 1938 Clinostomum complanatum, a trematode parasite new to man. Annotationes Zoologicae Japonenses, 17(3/4): 563-566.

YOSHIMURA, K.; ISHIGOOKA, S.; SATOH, I.; KAMEGAI, S. 1991 Clinostomum complanatum from the Pharynx of a Woman in Akita, Japan a case report. Japanese Journal of Parasitology, 40(1): 99-101.

ZEISS, C. 2012 AxioVision user’s guide, Release 4.8.2 – SP2 [software].Germany: Carl Zeiss. 1015p.
Published
2018-12-19
How to Cite
POZZA, Alice et al. Clinostomum sp. (Digenea: Clinostomidae) and Ascocotyle sp. (Digenea: Heterophyidae): metacercariae with zoonotic potential in fishes from Tramandaí River basin, southern Brazil. Boletim do Instituto de Pesca, [S.l.], v. 44, n. 1, p. 105-109, dec. 2018. ISSN 1678-2305. Available at: <https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/1286>. Date accessed: 23 may 2022. doi: https://doi.org/10.20950/1678-2305.2018.303.
Section
Short Communication