Carrageenin induced inflammation in Piaractus mesopotamicus (Osteichthyes: Characidae) cultured in Brazil

  • Maurício Laterça Martins Departamento de Aqüicultura, CCA, Universidade Federal de Santa Catarina (UFSC) - SC, Brasil http://orcid.org/0000-0002-0862-6927
  • Flávio Ruas de Moraes Departamento de Patologia Veterinária, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brasil http://orcid.org/0000-0001-6607-8650
  • Rodrigo Yudi Fujimoto Núcleo de Estudos Costeiros, Universidade Federal do Pará (UFPA), Campus de Bragança - Belém, PA, Brasil
  • Eduardo Makoto Onaka Instituto de Pesca, Centro Avançado de Pesquisa Tecnológica do Agronegócio do Pescado Continental - São José do Rio Preto, SP, Brasil http://orcid.org/0000-0003-2539-0036
  • Fabiana Rizzi Bozzo Departamento de Patologia Veterinária, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brasil
  • Julieta Rodini Engrácia de Moraes Departamento de Patologia Veterinária, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brasil http://orcid.org/0000-0002-8384-8781

Abstract

The present work evaluated the acute inflammatory response induced by carrageenin (500 µg) injection into the swim bladder of pacu (Piaractus mesopotamicus) after consecutive stress of capture. Fish were subjected to air exposition of 30 seconds repeatedly at 60 minutes intervals over six hours. The cellular inflammatory component was evaluated six hours after either the carrageenan or the control (saline injection) by total and differential counts. Cortisol and glucose were analyzed each hour. In the inflammatory exudate, thrombocytes were the most frequently observed cells, followed by macrophages. A significant reduction of cortisol levels was observed after the third stress of capture, as well as an increase in glucose after the second stimulus of stress. Differential counts of circulating leucocytes and thrombocytes show increased percentage of special granulocytic cells and a significant neutrophilia in stressed fish. Non-stressed fish injected with carrageenin presented neutrophilia and lymphopenia. In the swim bladder, carrageenin provoked congestion, interstitial hemorrhage, dissociation of the collagen sheaf and inflammatory infiltrate.


 

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Published
2018-10-29
How to Cite
MARTINS, Maurício Laterça et al. Carrageenin induced inflammation in Piaractus mesopotamicus (Osteichthyes: Characidae) cultured in Brazil. Boletim do Instituto de Pesca, [S.l.], v. 32, n. 1, p. 31-39, oct. 2018. ISSN 1678-2305. Available at: <https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/713>. Date accessed: 18 may 2022.