Ankistrodesmus gracilis(Reisch) Korsikov (Chlorophyta) laboratory cultured in CHU12 and macrophyte with NPK media

  • Lúcia Helena SIPAÚBA-TAVARES Universidade Estadual Paulista (UNESP), Centro de Aqüicultura
  • Luiz Carlos Coral I BARRA Universidad de Nariño, Facultad de Ciências Pecuarias, Ciudad Universitaria Torobajo, San Juan de Pasto, Nariño, Colombia
  • Tatiana Betioli FIORESI Universidade Estadual Paulista (UNESP), Centro de Aqüicultura


Two different culture media, namely CHU12 and inorganic fertilizer NPK (20-5-20) associated with macrophyte (Eichhornia crassipes) extract, were used to evaluate the development of A. gracilis. Growth rate, development, nutritional value and medium water quality were analyzed. A. gracilis in macrophyte+NPK medium had mean cell density (333 x 105 cells/mL) higher than medium CHU12 (302 x 105 cells/mL). Protein rate (12.52%PS), dry weight (397 x 107 pg/cell) and ashes (4.08 x 107 pg/cell) in A. gracilis was higher (p<0.05) than medium macrophyte+NPK. On the other hand, lipids, carbohydrates and fibers had the same rate (p>0.05) in both media. When hydrological variables in the culture medium of A. gracilis are taken into account, dissolved oxygen and free CO2 alone failed to have any significant difference (p>0.05) between the media employed. A. gracilis in the macrophyte+NPK medium had the same nutritional rate as the commercial medium CHU12, with excellent results for alga growth. Macrophyte medium may be used for large scale alga culture in the feed of fish larvae.


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How to Cite
SIPAÚBA-TAVARES, Lúcia Helena; BARRA, Luiz Carlos Coral I; FIORESI, Tatiana Betioli. Ankistrodesmus gracilis(Reisch) Korsikov (Chlorophyta) laboratory cultured in CHU12 and macrophyte with NPK media. Boletim do Instituto de Pesca, [S.l.], v. 35, n. 1, p. 111 - 118, nov. 2018. ISSN 1678-2305. Available at: <>. Date accessed: 04 july 2022.