Evaluation of 2-phenoxyethanol and menthol as anaesthetic agent in tilapia

  • Raquel de Andrade MELLO Mestranda. Universidade Federal de Lavras, Departamento de Ciências Veterinárias – DMV/UFLA
  • Leandro Santos Costa Aluno de graduação em Zootecnia. Universidade Federal de Lavras, Departamento de Zootecnia – DZO/UFLA
  • Daniel OKAMURA Pós-doutorando da Universidade Federal de Lavras, Departamento de Zootecnia – DZO/UFLA
  • Felipe Guedes de ARAÚJO Aluno de doutorado do curso de Zootecnia da Universidade Federal de Lavras, Departamento de Zootecnia – DZO/UFLA http://orcid.org/0000-0002-7394-4657
  • Paula Adriane Perez RIBEIRO Professora adjunta. Universidade Federal de Minas Gerais – UFMG
  • Fábio Mathias CORRÊA Professor assistente. Universidade Estadual de Santa Cruz – UESC
  • Priscila Vieira e ROSA Professora Associada. Universidade Federal de Lavras http://orcid.org/0000-0002-0052-079X

Abstract

This study aimed to determine the induction and recovery time in Nile tilapia (Oreochromis niloticus) submitted to the anaesthetic agents 2-phenoxyethanol and menthol. Five concentrations were evaluated of the 2 phenoxyethanol (0.45 mL L-1; 0.60 mL L-1; 0.75 mL L-1; 0.90 mL L-1; 1.05 mL L-1) and menthol (50 mg L-1; 75 mg L-1; 100 mg L-1; 125 mg L-1; 150 mg L-1) in masculinized juvenile tilapia. The experiments were conducted independently in a completely randomized design (CRD), consisting of five treatments for each anaesthetic and 20 repetitions (fish) per treatment. The induction and recovery from anesthesia were divided into three stages, according to the fishes' behavior under the effect of the anesthetic, recording the length of time for each stage. Limnological parameters of water were controlled (pH and dissolved oxygen). Statistical analysis was performed using a generalized linear model with gamma distribution. From this study, it is possible to conclude that, for Nile tilapia juvenile under the same conditions of maintenance, with increasing concentration of 2-phenoxyethanol and menthol there is a reduction in the time of anesthetic induction and recovery. Concentrations of 0.90 mL L-1 of 2-phenoxyethanol and 125 mg L-1 of menthol were suitable for anesthetic induction in tilapia.


 

References

FAÇANHA, M.F. e GOMES, L.C. 2005 A eficácia do mentol como anestésico para tambaqui (Colossoma macropomum, Characiformes: Characidae). Acta Amazônica, Manaus, 35(1): 71-75.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS (FAO). 2008 The State of World Fisheries and Aquaculture (SOFIA).Disponível em: http://www.fao.org/fishery/sofia/en Acesso em: 23 set. 2009.

GONÇALVES, A.F.N.; SANTOS, E.C.C.;FERNANDES, J.B.K.; TAKAHASHI, L.S. 2008
Mentol e eugenol como substitutos da benzocaína na indução anestésica de juvenis de pacu. Acta Scientiarum. Animal Sciences, Maringá,30(3): 339-344.

HIKASA, Y.; TAKASE, K.; OGASAWARA, T.;OGASAWARA, S. 1986 Anesthesia and
recovery with tricaine methanesulfonate,eugenol and thiopental sodium in the carp.
Cyprinus Carpio. Nippon Juigaku Zasshi, Towada,48(2): 341-351.

HISANO, H; ISHIKAWA, M.M; FERREIRA, R.A.;BULGARELLI, A.L.A; COSTA, T.R.; PADUA,
S.B. 2008 Tempo de indução e de recuperação de dourados Salminus brasiliensis (Cuvier, 1916) submetidos a diferentes concentrações com óleo de cravo Eugenia sp. Acta Scientiarum: Biological Sciences, Maringá, 30(3):303-307.

HOSKONEN, P. e PIRHONEN, J. 2004 Temperature effects on anaesthesia with clove oil in six temperate-zone fishes. Journal of Fish Biology,Scotland, 64: 1136–1142.

KEENE, J.I.; NOAKES, D.I.G.; MOCCIA, R.D.; SOTO, C.G. 1998 The efficacy of clove oil as an anaesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, Oxford,29: 89-101.

KING, W.V.; HOOPER, B.; HILLSGROVE, S.;BENTON, C.; BERLINSKY, D.L. 2005 The use
of clove oil, metomidate, tricaine methanesulphonate and 2-phenoxyethanol for
inducing anaesthesia and their effect on the cortisol stress response in black sea bass (Centropristis striata L.). Aquaculture Research,Oxford, 36: 1442-1449.

MUSSHOFF, U.; MADEJA, M.; BINDING, N.; WITTING, U.; SPECKMANN, E.J. 1999 Effects of 2-phenoxyethanol on N-methyl-D-aspartate (NMDA) receptor-mediated ion currents.Archives of Toxicology, Germany, 73: 55–59.

MYLONAS, C.; CARDINALETTI, G.; SIGELAKI, I.;POLZONETTIMAGNI, A. 2005 Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass and gilthead sea bream at different temperatures. Aquaculture, Amsterdam, 246(1-4):467-481.

OKAMURA, D.; ARAÚJO, F.G.; ROSA, P.V.;FREITAS, R.T.F.; MURGAS, L.D.S.; CESAR, M.P.
2010 Influência da concentração de benzocaína e do comprimento dos peixes na anestesia e na recuperação de tilápias-do-nilo. Revista Brasileira de Zootecnia, Viçosa, 39(5): 971-976.

R Development Core Team . 2011 R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna,Austria. Disponível em: http://www.Rproject.org

ROSS, L.G. e ROSS, B. 2008 Anaesthetic and sedative techniques for aquatic animals. 3ª ed. Oxford:Blackwell Science, 236p.

ROUBACH, R. e GOMES, L.C. 2001 O uso de anestésicos durante o manejo de peixes. Panorama da Aquicultura, Rio de Janeiro, 11(66): 37-40.

SIMÕES, L.N. e GOMES, L.C. 2009 Eficácia do mentol como anestésico para juvenis de tilápiado-nilo (Oreochromis niloticus). Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, 61(3): 613-620.

UETA, K.; SUZUKI, T.; SUGIMOTO, M.; UCHIDA,I.; MASHIMO, T. 2007 Local anesthetics have different mechanisms and sites of action at recombinant 5-HT3 receptors. Regional Anesthesia and Pain Medicine, Schaumburg, 32: 462–470.

VELÍŠEK, J.; WLASOW T.; GOMULKA, P.;SVOBODOVA, Z.; NOVOTNY, L. 2007 Effects of
2- anaesthesia on sheatfish (Silurus glanis L.).Veterinarni Medicina, Czech, 52(3): 103-110.

WATT, E.E.; BETTS, B.A.; KOTEY, F.O.; HUMBERT,D.J.; GRIFFITH, T.N.; KELLY, E.W.; VENESKEY, K.C.; GILL, N.; ROWAN, K.C.; JENKINS, A.;HALL, A.C. 2008 Menthol shares general anesthetic activity and sites of action on the GABAA receptor with the intravenous agent, propofol. European Journal of Pharmacology,Netherlands, 590(1/3): 120–126.

WEBER, R.A.; PELETEIRO, J.B.; GARCÍA MARTÍN,L.O.; ALDEGUNDE, M. 2009 The efficacy of 2-phenoxyethanol, metomidate, clove oil and MS222 as anaesthetic agents in the Linguado (Solea senegalensis Kaup 1858). Aquaculture,Amsterdam, 288: 147–150.

ZAHL, I.H.; KIESSLING, A.; SAMUELSEN, O.B.;HANSEN, M.K. 2009 Anaesthesia of Atlantic cod (Gadus morhua) — Effect of pre-anaesthetic sedation, and importance of body weight, temperature and stress. Aquaculture,Amsterdam, 295: 52–59.
Published
2018-11-09
How to Cite
MELLO, Raquel de Andrade et al. Evaluation of 2-phenoxyethanol and menthol as anaesthetic agent in tilapia. Boletim do Instituto de Pesca, [S.l.], v. 38, n. 1, p. 53 - 59, nov. 2018. ISSN 1678-2305. Available at: <https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/942>. Date accessed: 30 june 2022.